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prs404 ptef1 ago1  (Addgene inc)


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    Structured Review

    Addgene inc prs404 ptef1 ago1
    Prs404 Ptef1 Ago1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prs404+ptef1+ago1/pRS404-PTEF-Ago1+(Plasmid+%2322313)/pmc05162094-203-85-86
    Average 93 stars, based on 11 article reviews
    prs404 ptef1 ago1 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Can terminators be used as insulators into yeast synthetic gene circuits?
    Article Snippet: .. The yeast integrative shuttle-vector plasmid pRSII406 (Addgene-35442, a gift from Steven Haase) [ ] was used as a backbone for the construction of every transcription unit. pGPD, pACT1, the CYC1 promoter (pCYC1), and the genomic CYC1 terminator (genCYC1t) were extracted from the yeast S. cerevisiae genome (strain FY1679-08A, see below) following the procedure in [ ]. pCYC1 served as a template to PCR out the sequence of pCYC1noTATA and pCYC1min. pTEF2 was obtained from the MIT Registry part BBa_K801010, pTEF1 was extracted via PCR from pRS404-pTEF1-Ago1 (Addgene-22313, a gift from David Bartel), pADH1 was obtained from pHCA/GAL4(1-93).ER.VP16 [ ] (courtesy of Picard lab, University of Geneva, Switzerland). .. Every transcription unit expresses either the yomKate2 red fluorescent protein obtained from pFA6a-link-yomKate2-CaURA3 (Addgene-44878, a gift from Wendell Lim and Kurt Thorn) or the yeast enhanced green fluorescent protein (yEGFP) obtained from pRS31-glag [ ] (courtesy of Hasty lab, University of California, San Diego, USA).

    Polymerase Chain Reaction:

    Article Title: Can terminators be used as insulators into yeast synthetic gene circuits?
    Article Snippet: .. The yeast integrative shuttle-vector plasmid pRSII406 (Addgene-35442, a gift from Steven Haase) [ ] was used as a backbone for the construction of every transcription unit. pGPD, pACT1, the CYC1 promoter (pCYC1), and the genomic CYC1 terminator (genCYC1t) were extracted from the yeast S. cerevisiae genome (strain FY1679-08A, see below) following the procedure in [ ]. pCYC1 served as a template to PCR out the sequence of pCYC1noTATA and pCYC1min. pTEF2 was obtained from the MIT Registry part BBa_K801010, pTEF1 was extracted via PCR from pRS404-pTEF1-Ago1 (Addgene-22313, a gift from David Bartel), pADH1 was obtained from pHCA/GAL4(1-93).ER.VP16 [ ] (courtesy of Picard lab, University of Geneva, Switzerland). .. Every transcription unit expresses either the yomKate2 red fluorescent protein obtained from pFA6a-link-yomKate2-CaURA3 (Addgene-44878, a gift from Wendell Lim and Kurt Thorn) or the yeast enhanced green fluorescent protein (yEGFP) obtained from pRS31-glag [ ] (courtesy of Hasty lab, University of California, San Diego, USA).

    Sequencing:

    Article Title: Can terminators be used as insulators into yeast synthetic gene circuits?
    Article Snippet: .. The yeast integrative shuttle-vector plasmid pRSII406 (Addgene-35442, a gift from Steven Haase) [ ] was used as a backbone for the construction of every transcription unit. pGPD, pACT1, the CYC1 promoter (pCYC1), and the genomic CYC1 terminator (genCYC1t) were extracted from the yeast S. cerevisiae genome (strain FY1679-08A, see below) following the procedure in [ ]. pCYC1 served as a template to PCR out the sequence of pCYC1noTATA and pCYC1min. pTEF2 was obtained from the MIT Registry part BBa_K801010, pTEF1 was extracted via PCR from pRS404-pTEF1-Ago1 (Addgene-22313, a gift from David Bartel), pADH1 was obtained from pHCA/GAL4(1-93).ER.VP16 [ ] (courtesy of Picard lab, University of Geneva, Switzerland). .. Every transcription unit expresses either the yomKate2 red fluorescent protein obtained from pFA6a-link-yomKate2-CaURA3 (Addgene-44878, a gift from Wendell Lim and Kurt Thorn) or the yeast enhanced green fluorescent protein (yEGFP) obtained from pRS31-glag [ ] (courtesy of Hasty lab, University of California, San Diego, USA).



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    prs404 ptef1 ago1  (Addgene inc)
    93
    Addgene inc prs404 ptef1 ago1
    Prs404 Ptef1 Ago1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prs404+ptef1+ago1/pRS404-PTEF-Ago1+(Plasmid+%2322313)/pmc05162094-203-85-86
    Average 93 stars, based on 1 article reviews
    prs404 ptef1 ago1 - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

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